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We have started planning of the Sveta’s work during her time in Gissen. We need your part in it. Sveta will bring oligos. Please, order the (ICH2CO)2O (number in Sigma catalog 284262-250MG). 3 packaging by 250 mg of it will be quite enough for the first time. Sveta will purify good proteins and will do crosslinking reaction. She hope reach high yield of conjugate (she has had yield about 35-40% with nickase BspD6I). About crystallization: Do you have some ideas for taking pure and in native form conjugate? As Roger said, increasing concentration of KCl till 200 mM is dissociated the complex between MutS and the DNA. Does MutS keep its native structure in this condition? We want know your vision about Sveta’s time in Gissen. Please, write us about it.
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